Cyclic Nucleotide Binding GAF Domains from Phosphodiesterases: Structural and Mechanistic Insights

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MINIREVIEW Properties and Functions of GAF Domains in Cyclic Nucleotide Phosphodiesterases and Other Proteins

This review focuses primarily on our current understanding of the structure and function of GAF domains in cyclic nucleotide phosphodiesterases (PDEs). The GAF domain was originally identified by Aravind and Ponting (1997) using the position-specific iterative BLAST method (Altschul et al., 1997). The consensus sequence defining GAF domains is taken from a characteristic primary sequence of che...

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This review focuses primarily on our current understanding of the structure and function of GAF domains in cyclic nucleotide phosphodiesterases (PDEs). The GAF domain was originally identified by Aravind and Ponting (1997) using the position-specific iterative BLAST method (Altschul et al., 1997). The consensus sequence defining GAF domains is taken from a characteristic primary sequence of che...

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GAF domains are a large family of regulatory domains, and a subset are found associated with enzymes involved in cyclic nucleotide (cNMP) metabolism such as adenylyl cyclases and phosphodiesterases. CyaB2, an adenylyl cyclase from Anabaena, contains two GAF domains in tandem at the N-terminus and an adenylyl cyclase domain at the C-terminus. Cyclic AMP, but not cGMP, binding to the GAF domains ...

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ژورنال

عنوان ژورنال: Structure

سال: 2009

ISSN: 0969-2126

DOI: 10.1016/j.str.2009.07.019